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Literature summary for 6.1.1.11 extracted from
- Differential modes of transfer RNASer recognition in Methanosarcina barkeri (2004), J. Biol. Chem., 279, 48780-48786.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values for different tRNASer variants | Methanosarcina barkeri | |
| 0.0013 | - |
tRNASer(GCU) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 0.0026 | - |
tRNASer(GGA) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 0.0029 | - |
tRNASer(CGA) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 0.003 | - |
tRNASer(CGA) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri | |
| 0.0047 | - |
tRNASer(GGA) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri | |
| 0.0053 | - |
tRNASer(GCU) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | reaction with tRNASer variants. Two dissimilar seryl-transfer RNA synthases exist in Methanosarcina barkeri, one of bacterial type and the other resembling SerRS present only in some methanogenic archaea. The anticodon stem base pair G30:C40, a determinant for the specific tRNASer recognition, contributes to the efficiency of serylation in both seryl-transfer RNA synthases. The two seryl-transfer RNA synthases do not possess a uniform mode of tRNASer recognition. The methanogenic seryl-transfer RNA synthase relies on G1:C72 identity and on the number of unpaired nucleotides at the base of the variable stem for tRNASer recognition, unlike its bacterial type counterpart | Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? |  |
| ATP + L-serine + tRNASer(CGA) | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer(CGA) | - |
? | |
| ATP + L-serine + tRNASer(GCU) | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer(GCU) | - |
? | |
| ATP + L-serine + tRNASer(GGA) | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer(GGA) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterial SerRS | - |
Methanosarcina barkeri |
| methanogenic SerRS | - |
Methanosarcina barkeri |
| SerRS | - |
Methanosarcina barkeri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover numbers for different tRNASer variants | Methanosarcina barkeri | |
| 0.9 | - |
tRNASer(GGA) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 0.92 | - |
tRNASer(GCU) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 1.07 | - |
tRNASer(CGA) | pH 7.2, 37°C, bacterial type SerRS | Methanosarcina barkeri | |
| 1.13 | - |
tRNASer(GCU) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri | |
| 3.05 | - |
tRNASer(CGA) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri | |
| 4.4 | - |
tRNASer(GGA) | pH 7.2, 37°C, methanogenic SerRS | Methanosarcina barkeri |
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