Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Heckl, M.; Busch, K.; Gross, H.J.
    Minimal tRNASer and tRNASec substrates for human seryl-tRNA synthetase: contribution of tRNA domains to serylation and tertiary structure (1998), FEBS Lett., 427, 315-319.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information deletion of any but the anticodon domain of tRNASer and tRNASec causes a dramatic loss of serine acceptance Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00054
-
tRNASer pH 7.6, 37°C Homo sapiens
0.0011
-
tRNASer mutant without anticodon arm pH 7.6, 37°C Homo sapiens
0.0033
-
tRNASec pH 7.6, 37°C Homo sapiens
0.004
-
tRNASec mutant without anticodon arm pH 7.6, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Homo sapiens
-
AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
class II enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
wild-type and mutant tRNA substrates, relative activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASec selenocysteine-incorporating tRNA wild-type and deletion mutants, secondary structures Homo sapiens AMP + diphosphate + L-seryl-tRNASec
-
?
ATP + L-serine + tRNASec mutant without anticodon arm
-
Homo sapiens ?
-
?
ATP + L-serine + tRNASer
-
Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer 4 isoacceptors of tRNASer, and tRNASer deletion mutants, secondary structures, the anticodon arms D and T are not involved in tRNA substrate recognition by the enzyme, only in formation of the L-shaped tRNA structure Homo sapiens AMP + diphosphate + L-seryl-tRNASer
-
?
ATP + L-serine + tRNASer mutant without anticodon arm
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
62 kDa RNA-binding protein
-
Homo sapiens
Serine transfer RNA synthetase
-
Homo sapiens
Serine translase
-
Homo sapiens
Serine--tRNA ligase
-
Homo sapiens
SerRS
-
Homo sapiens
SerRSmt
-
Homo sapiens
SERSEC
-
Homo sapiens
Seryl-transfer ribonucleate synthetase
-
Homo sapiens
Seryl-transfer ribonucleic acid synthetase
-
Homo sapiens
Seryl-transfer RNA synthetase
-
Homo sapiens
Seryl-tRNA synthetase
-
Homo sapiens
Synthetase, seryl-transfer ribonucleate
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
tRNASec mutant without anticodon arm pH 7.6, 37°C Homo sapiens
0.007
-
tRNASec pH 7.6, 37°C Homo sapiens
0.023
-
tRNASer mutant without anticodon arm pH 7.6, 37°C Homo sapiens
0.062
-
tRNASer pH 7.6, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens