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Literature summary for 6.1.1.10 extracted from

  • Lee, J.Y.; Kim, D.G.; Kim, B.G.; Yang, W.S.; Hong, J.; Kang, T.; Oh, Y.S.; Kim, K.R.; Han, B.W.; Hwang, B.J.; Kang, B.S.; Kang, M.S.; Kim, M.H.; Kwon, N.H.; Kim, S.
    Promiscuous methionyl-tRNA synthetase mediates adaptive mistranslation to protect cells against oxidative stress (2014), J. Cell Sci., 127, 4234-4245 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
S209A/S825A the mutant shows minimal phosphorylation upon incubation with extracellular signal-related kinase and leads to reduced activity compared to the wild type enzyme Escherichia coli
S209D/S825D the mutation mimicks dual phosphorylation and leads to reduced activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00303
-
 L-methionyl-tRNAMet wild type enzyme, at pH 7.4 and 37°C Escherichia coli
0.00796
-
 L-methionyl-tRNAMet mutant enzyme S209A/S825A, at pH 7.4 and 37°C Escherichia coli
0.04183
-
 L-methionyl-tRNAMet mutant enzyme S209D/S825D, at pH 7.4 and 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated at Ser209 and Ser825 by extracellular signal-related kinase during oxidative stress Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + diphosphate + L-methionyl-tRNAMet
-
 Escherichia coli ATP + L-methionine + tRNAMet
-
 r
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 r
additional information no activity with L-methionyl-tRNALys Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Escherichia coli
MRS
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
 L-methionyl-tRNAMet mutant enzyme S209A/S825A, at pH 7.4 and 37°C Escherichia coli
0.127
-
 L-methionyl-tRNAMet mutant enzyme S209D/S825D, at pH 7.4 and 37°C Escherichia coli
0.157
-
 L-methionyl-tRNAMet wild type enzyme, at pH 7.4 and 37°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function enzyme-mediated methionine-misacylation reduces intracellular reactive oxygen species levels and protects cells from oxidative damage Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 L-methionyl-tRNAMet mutant enzyme S209A/S825A, at pH 7.4 and 37°C Escherichia coli
3.1
-
 L-methionyl-tRNAMet mutant enzyme S209D/S825D, at pH 7.4 and 37°C Escherichia coli
51
-
 L-methionyl-tRNAMet wild type enzyme, at pH 7.4 and 37°C Escherichia coli