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Literature summary for 6.1.1.10 extracted from

  • Jones, T.E.; Alexander, R.W.; Pan, T.
    Misacylation of specific nonmethionyl tRNAs by a bacterial methionyl-tRNA synthetase (2011), Proc. Natl. Acad. Sci. USA, 108, 6933-6938.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C477S the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme Escherichia coli
D369A the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme Escherichia coli
F277L the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme Escherichia coli
Q211 the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme Escherichia coli
Q213A the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme Escherichia coli
T489A the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme Escherichia coli
W221A the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme Escherichia coli
W461D the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme Escherichia coli
Y490A the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00959
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAArg(CCU) tRNA mismethionylation Escherichia coli ?
-
 ?
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAThr(CGU) tRNA mismethionylation Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Escherichia coli
MRS
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli