Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C477S | the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
D369A | the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
F277L | the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
Q211 | the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
Q213A | the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
T489A | the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
W221A | the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
W461D | the mutant displays at least a 6fold reduction in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
Y490A | the mutant shows at least a 2fold increase in mismethionylation percentage compared to the wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Escherichia coli | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00959 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAArg(CCU) | tRNA mismethionylation | Escherichia coli | ? | - |
? | |
ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAThr(CGU) | tRNA mismethionylation | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Escherichia coli |
MRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |