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Literature summary for 6.1.1.10 extracted from

  • Schmitt, E.; Tanrikulu, I.C.; Yoo, T.H.; Panvert, M.; Tirrell, D.A.; Mechulam, Y.
    Switching from an induced-fit to a lock-and-key mechanism in an aminoacyl-tRNA synthetase with modified specificity (2009), J. Mol. Biol., 394, 843-851.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
the unmutated monomeric versions of MetRS, truncated at residue 548, M547, is produced from the plasmid pBSM547, the pMTY21-sll plasmid codes for an N-terminally 6xHis-tagged M547 MetRS, leading to expression of the MetRS-SLL mutant Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of the mutated MetRS is determined in the apo form as well as complexed with methionine or azidonorleucine to 1.4 to 1.7 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
L13S/Y260L/H301L MetRS SLL-mutant with modified substrate specificity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00959
-
-

Purification (Commentary)

Purification (Comment) Organism
the SLL-mutant is purified on columns containing Talon affinity resin and Q-Hiload resin, respectively Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + azidonorleucine + tRNAMet MetRS SLL-mutant Escherichia coli AMP + diphosphate + azidonorleucinyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Escherichia coli
MetRS
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli