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Literature summary for 6.1.1.10 extracted from

  • Fasiolo,F.; Gibson, B.W.; Walter, P.; Chatton, B.; Biemann, K.; Boulanger, Y.
    Cytoplasmic methionyl-tRNA synthetase from bakers' yeast. A monomer with a post-translationally modified N-terminus (1985), J. Biol. Chem., 260, 15571-15576.
    View publication on PubMed
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information a strain carrying the MES1 structure gene on a high copy number plasmid, pFL1 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 tRNAMet
-
 Saccharomyces cerevisiae
0.005
-
 Met
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 a strain carrying the MES1 structure gene on a high copy number plasmid (pFL1)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Saccharomyces cerevisiae AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 Met-tRNAMet
-
 Saccharomyces cerevisiae