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Literature summary for 6.1.1.10 extracted from
- Characterization of the human mitochondrial methionyl-tRNA synthetase (2004), Biochemistry, 43, 9743-9754.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | bovine serum albumin has no effect on the enzyme | Homo sapiens | |
| spermine | slight stimulation, additionally spermine increases the enzyme activity by stabilizing conformation of negatively charged DNA | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mature enzyme form, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin has no effect on the enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00015 | - |
tRNAMet | pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme | Homo sapiens | |
| 0.00016 | - |
tRNAMet | pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme | Homo sapiens | |
| 0.0021 | - |
tRNAMet | pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme | Homo sapiens | |
| 0.018 | - |
L-methionine | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
| 0.085 | - |
ATP | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | mtMetRS | Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulates | Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Homo sapiens | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P56192 | class I enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged mature enzyme form from Escherichia coli by ion exchange chromatography | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet | two-step reaction process via anenzyme-L-Met-AMP intermediate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
| ATP + L-methionine + tRNAMet | human mitochondrial wild-type and mutant mtRNAs: T5048 deletion, and T5052C or T5012A point mutations, initiator tRNA from Escherichia coli, tRNAMet from Bos taurus | Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
| additional information | the enzyme performs ATP/diphosphate exchange in absence of substrates | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67000, SDS-PAGE | Homo sapiens |
| More | domain structure, structure modeling based on crystal structures of Escherichia coli with PDB code 1QQR, overview, the enzyme contains the consensus motifs of class I aminoacyl-tRNA, but lacks the Zn2+ binding motif and the C-terminal dimerization region | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionyl-tRNA synthetase | - |
Homo sapiens |
| MetRS | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
tRNAMet | pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme | Homo sapiens | |
| 0.021 | - |
tRNAMet | pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme | Homo sapiens | |
| 0.033 | - |
ATP | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
| 0.41 | - |
L-methionine | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
| 11 | - |
tRNAMet | pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.2 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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