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Literature summary for 6.1.1.10 extracted from
- A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana (1998), Proc. Natl. Acad. Sci. USA, 95, 11014-11019.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA determination and analysis, overexpression in Escherichia coli SG13009, in vitro translation, expression of the enzyme as GFP-fusion protein in Nicotiana tabacum suspension cells | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme, possessing a targeting signal sequence with dual function | Arabidopsis thaliana | 9507 | - |
| chloroplast | the isozyme from chloroplasts is identical with the one from mitochondria | Pisum sativum | 9507 | - |
| cytoplasm | cytoplasmic enzyme form | Pisum sativum | 5737 | - |
| mitochondrion | a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme, possessing a targeting signal sequence with dual function | Arabidopsis thaliana | 5739 | - |
| mitochondrion | the isozyme from mitochondria is identical with the one from chloroplasts | Pisum sativum | 5739 | - |
| additional information | the in vitro synthesized enzyme is taken up by specific transport into chloroplasts of Pisum sativum, and in mitochondria of Solanum tuberosum, the imported protein becomes processed in both cases | Arabidopsis thaliana | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, processed enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Pisum sativum | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |  |
| ATP + L-methionine + tRNAMet | Arabidopsis thaliana | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme | - |
| Arabidopsis thaliana | O23761 | a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme | - |
| Arabidopsis thaliana | Q9M2T9 | a single gene of chloroplast origin codes for mitochondrial and chloroplastic isozyme | - |
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzymes from chloroplasts and mitochondria, which are indistinguishable in the purificaion process, partial | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Pisum sativum | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | - |
Arabidopsis thaliana | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, processed enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methionine translase | - |
Pisum sativum |
| Methionine translase | - |
Arabidopsis thaliana |
| Methionine--tRNA ligase | - |
Pisum sativum |
| Methionine--tRNA ligase | - |
Arabidopsis thaliana |
| Methionyl tRNA synthetase | - |
Pisum sativum |
| Methionyl tRNA synthetase | - |
Arabidopsis thaliana |
| Methionyl-transfer ribonucleate synthetase | - |
Pisum sativum |
| Methionyl-transfer ribonucleate synthetase | - |
Arabidopsis thaliana |
| Methionyl-transfer ribonucleic acid synthetase | - |
Pisum sativum |
| Methionyl-transfer ribonucleic acid synthetase | - |
Arabidopsis thaliana |
| Methionyl-transfer RNA synthetase | - |
Pisum sativum |
| Methionyl-transfer RNA synthetase | - |
Arabidopsis thaliana |
| MetRS | - |
Pisum sativum |
| MetRS | - |
Arabidopsis thaliana |
| Synthetase, methionyl-transfer ribonucleate | - |
Pisum sativum |
| Synthetase, methionyl-transfer ribonucleate | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pisum sativum | |
| ATP | - |
Arabidopsis thaliana | |
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