Literature summary for 6.1.1.10 extracted from

Meinnel, T.; Mechulam, Y.; Dardel, F.; Schmitter, J.M.; Hountondji, C.; Brunie, S.; Dessen, P.; Fayat, G.; Blanquet, S.
Methionyl-tRNA synthetase from E. coli - A review (1990), Biochimie, 72, 625-632.

Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
truncated form	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K335Q	mutants produced by site-directed mutagenesis, Lys335-Gln substitution results in a complete loss of activity, similar loss of activity is observed when Lys335 is changed into alanine, glutamic acid, or arginine	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Periodate-oxidized ATP	-	Escherichia coli
Periodate-oxidized tRNA	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-values of mutant enzymes in aminoacylation	Escherichia coli
0.0009	-	tRNAMet	wild-type enzyme	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	2 tightly bound Zn2+ per polypeptide chain, in the vicinity of the active site	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
76000	-	2 * 76000	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	wild-type enzyme and mutants produced by site-directed mutagenesis	-

Renatured (Commentary)

Renatured (Comment)	Organism
facile, after unfolding with 5 M guanidine under conditions where zinc is not sequestred	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-methionine + tRNAMet	-	Escherichia coli	AMP + diphosphate + L-methionyl-tRNAMet	-	?
additional information	methionine-dependent ATP-diphosphate exchange	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 76000	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	turnover numbers of mutant enzymes	Escherichia coli
additional information	-	additional information	in aminoacylation	Escherichia coli
3.3	-	tRNAMet	wild-type enzyme	Escherichia coli

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Release 2023.1 (January 2023)