Cloned (Comment) | Organism |
---|---|
TyrRS, phylogenetic analysis, the viral aminoacyl tRNA synthetases have not been acquired recently by horizontal gene transfer from a cellular host but rather militate in favor of an intricate evolutionary relationship between large DNA viruses and ancestral eukaryotes | Acanthamoeba polyphaga Mimivirus |
Crystallization (Comment) | Organism |
---|---|
purified selenomethionyl-TyrRSapm in complex with tyrosinol, crystallization is improved by introducing anion-exchange chromatography, vapour diffusion method, 0.002 ml of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.4, 25°C, is mixed with 500 nl reservoir solution containing 0.1 M sodium citrate, pH 5.5, and 6-9% PEG 4000 w/v, 15% 2-methyl-2,4-pentane-d12-diol, 0.1 M KCl, 1 mM MgCl2, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement | Acanthamoeba polyphaga Mimivirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview | Acanthamoeba polyphaga Mimivirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acanthamoeba polyphaga Mimivirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Acanthamoeba polyphaga Mimivirus | the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
additional information | Acanthamoeba polyphaga Mimivirus | mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba polyphaga Mimivirus | Q5UPJ7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview | Acanthamoeba polyphaga Mimivirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview | Acanthamoeba polyphaga Mimivirus | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr | functional idiosyncrasies of the viral TyrRS, activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview, the TyrRS specificity for tyrosine and recognition of the tRNATyr acceptor stem show conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview | Acanthamoeba polyphaga Mimivirus | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
additional information | mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas | Acanthamoeba polyphaga Mimivirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | dimer interface structure from crystal structure analysis, modeling, overview | Acanthamoeba polyphaga Mimivirus |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Acanthamoeba polyphaga Mimivirus |
TyrRS | - |
Acanthamoeba polyphaga Mimivirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acanthamoeba polyphaga Mimivirus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba polyphaga Mimivirus |