Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.1 extracted from

  • Park, Y.C.; Bedouelle, H.
    Dimeric tyrosyl-tRNA synthetase from Bacillus stearothermophilus unfolds through a monomeric intermediate. A quantitative analysis under equilibrium conditions (1998), J. Biol. Chem., 273, 18052-18059.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene tyrS, overexpression of wild-type and mutant enzymes from phage M13-BY(DELTA1) in Escherichia coli strains RZ1032 and TG2 Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
I52L unaltered enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, compensates mutation L105V partially, destabilized association between subunits Geobacillus stearothermophilus
L105V unaltered enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, destabilization of the monomeric intermediate of unfolding, mutation can be partially compensated by mutation I52L Geobacillus stearothermophilus
M55L increase in kinetic stability at 68.5°C compared to the wild-type, mutation is not coupled to others in its effects, slightly increased kinetic stability at 68.5°C Geobacillus stearothermophilus
additional information effects of coupling of mutations on thermal stability Geobacillus stearothermophilus
T51P increased enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, slightly destalized mutant Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
L-tyrosine mutant M55L, pH 7.8, 25°C Geobacillus stearothermophilus
0.0018
-
L-tyrosine mutant I52L, pH 7.8, 25°C Geobacillus stearothermophilus
0.0021
-
L-tyrosine wild-type enzyme and mutant L105V, pH 7.8, 25°C Geobacillus stearothermophilus
2
-
ATP mutant M55L, pH 7.8, 25°C Geobacillus stearothermophilus
2.1
-
ATP wild-type enzyme, pH 7.8, 25°C Geobacillus stearothermophilus
2.4
-
ATP mutant I52L, pH 7.8, 25°C Geobacillus stearothermophilus
2.7
-
ATP mutant L105V, pH 7.8, 25°C Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + tyrosine + tRNATyr Geobacillus stearothermophilus
-
AMP + Tyr-tRNATyr + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
mechanism of evolution
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Geobacillus stearothermophilus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + tyrosine + tRNATyr
-
Geobacillus stearothermophilus AMP + Tyr-tRNATyr + diphosphate
-
?

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
Geobacillus stearothermophilus
TyrRS
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
effects of mutations and of coupling of mutations on thermal parameters and stability, overview Geobacillus stearothermophilus
68.5
-
wild-type enzyme shows 50% inactivation after 27 min Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
L-tyrosine mutant M55L, pH 7.8, 25°C Geobacillus stearothermophilus
3.6
-
L-tyrosine mutant I52L, pH 7.8, 25°C Geobacillus stearothermophilus
4.6
-
ATP mutant M55L, pH 7.8, 25°C Geobacillus stearothermophilus
4.9
-
L-tyrosine mutant L105V, pH 7.8, 25°C Geobacillus stearothermophilus
5.4
-
L-tyrosine wild-type enzyme, pH 7.8, 25°C Geobacillus stearothermophilus
6.8
-
ATP mutant I52L, pH 7.8, 25°C Geobacillus stearothermophilus
7.7
-
ATP wild-type enzyme, pH 7.8, 25°C Geobacillus stearothermophilus
12
-
ATP mutant L105V, pH 7.8, 25°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Geobacillus stearothermophilus