Cloned (Comment) | Organism |
---|---|
gene tyrS, overexpression of wild-type and mutant enzymes from phage M13-BY(DELTA1) in Escherichia coli strains RZ1032 and TG2 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
I52L | unaltered enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, compensates mutation L105V partially, destabilized association between subunits | Geobacillus stearothermophilus |
L105V | unaltered enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, destabilization of the monomeric intermediate of unfolding, mutation can be partially compensated by mutation I52L | Geobacillus stearothermophilus |
M55L | increase in kinetic stability at 68.5°C compared to the wild-type, mutation is not coupled to others in its effects, slightly increased kinetic stability at 68.5°C | Geobacillus stearothermophilus |
additional information | effects of coupling of mutations on thermal stability | Geobacillus stearothermophilus |
T51P | increased enzyme activity in the diphosphate exchange reaction, decreased kinetic stability at 68.5°C compared to the wild-type, slightly destalized mutant | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
L-tyrosine | mutant M55L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
0.0018 | - |
L-tyrosine | mutant I52L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
0.0021 | - |
L-tyrosine | wild-type enzyme and mutant L105V, pH 7.8, 25°C | Geobacillus stearothermophilus | |
2 | - |
ATP | mutant M55L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
2.1 | - |
ATP | wild-type enzyme, pH 7.8, 25°C | Geobacillus stearothermophilus | |
2.4 | - |
ATP | mutant I52L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
2.7 | - |
ATP | mutant L105V, pH 7.8, 25°C | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | Geobacillus stearothermophilus | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
mechanism of evolution | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | - |
Geobacillus stearothermophilus | AMP + Tyr-tRNATyr + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
TyrRS | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
effects of mutations and of coupling of mutations on thermal parameters and stability, overview | Geobacillus stearothermophilus |
68.5 | - |
wild-type enzyme shows 50% inactivation after 27 min | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
L-tyrosine | mutant M55L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
3.6 | - |
L-tyrosine | mutant I52L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
4.6 | - |
ATP | mutant M55L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
4.9 | - |
L-tyrosine | mutant L105V, pH 7.8, 25°C | Geobacillus stearothermophilus | |
5.4 | - |
L-tyrosine | wild-type enzyme, pH 7.8, 25°C | Geobacillus stearothermophilus | |
6.8 | - |
ATP | mutant I52L, pH 7.8, 25°C | Geobacillus stearothermophilus | |
7.7 | - |
ATP | wild-type enzyme, pH 7.8, 25°C | Geobacillus stearothermophilus | |
12 | - |
ATP | mutant L105V, pH 7.8, 25°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |