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Literature summary for 5.6.2.4 extracted from
- Plasmodium falciparum specific helicase 3 is nucleocytoplasmic protein and unwinds DNA duplex in 3' to 5' direction (2017), Sci. Rep., 7, 13146 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Codon Plus cells | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | localized majorly in cytoplasm | Plasmodium falciparum | 5737 | - |
| nucleus | - |
Plasmodium falciparum | 5634 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8IAR1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Plasmodium falciparum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| schizont | - |
Plasmodium falciparum | - |
| trophozoite | - |
Plasmodium falciparum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| partial duplex DNA | 32P-labelled 47-mer DNA oligodeoxynucleotide annealed to M13mp19 single-stranded DNA. The enzyme has single-stranded DNA-dependent ATPase activity and DNA helicase activity in the 3' to 5' direction. The enzyme shows up to 58% unwinding with the 3' to 5' direction-specific substrate and is unable to show the activity with 5' to 3' direction-specific substrate | Plasmodium falciparum | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 157000, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| helicase 3 | - |
Plasmodium falciparum |
| PSH3 | - |
Plasmodium falciparum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | the enzyme shows maximum helicase activity in the presence of ATP (60%) | Plasmodium falciparum | |
| CTP | the enzyme shows low helicase activity in the presence of CTP (35%) | Plasmodium falciparum | |
| dATP | the enzyme shows maximum helicase activity in the presence of dATP (58%) | Plasmodium falciparum | |
| additional information | the enzyme exhibits considerably lower activitywith dGTP, GTP, dCTP, dTTP and UTP compared to ATP | Plasmodium falciparum |
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