Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21AI cells | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
K275A | inactive | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM used in assay conditions | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA-agarose column chromatography | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has DNA-dependent ATPase activity and helicase activity that proceeds in the 3'-5' direction and needs at least five 3' overhanging nucleotides. The enzyme is also able to unwind DNA structures including D-loops and Holliday junctions. The enzyme does not unwind blunt-ended DNA duplexes | Caenorhabditis elegans | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, His6-tagged enzyme, SDS-PAGE | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
BLM helicase | - |
Caenorhabditis elegans |
HIM-6 | - |
Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Caenorhabditis elegans |