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Literature summary for 5.6.2.4 extracted from

  • Jung, H.; Lee, J.; Choi, S.; Lee, H.; Ahn, B.
    Characterization of the Caenorhabditis elegans HIM-6/BLM helicase Unwinding recombination intermediates (2014), PLoS ONE, 9, e102402 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21AI cells Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
K275A inactive Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM used in assay conditions Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA-agarose column chromatography Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has DNA-dependent ATPase activity and helicase activity that proceeds in the 3'-5' direction and needs at least five 3' overhanging nucleotides. The enzyme is also able to unwind DNA structures including D-loops and Holliday junctions. The enzyme does not unwind blunt-ended DNA duplexes Caenorhabditis elegans ?
-
-

Subunits

Subunits Comment Organism
? x * 110000, His6-tagged enzyme, SDS-PAGE Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
BLM helicase
-
Caenorhabditis elegans
HIM-6
-
Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
ATP
-
Caenorhabditis elegans