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Literature summary for 5.6.2.4 extracted from
- Drosophila RecQ4 has a 3'-5' DNA helicase activity that is essential for viability (2009), J. Biol. Chem., 284, 30845-30852 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DNA | the presence of DNA greatly stimulates the hydrolysis of ATP | Drosophila melanogaster |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 insect cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K898N | ATPase-dead mutant which does not exhibit helicase activity | Drosophila melanogaster |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 8 mM used in assay conditions | Drosophila melanogaster | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose column chromatography | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme has a DNA-dependent ATPase activity and is a 3'-5' DNA helicase dependent on hydrolysis of ATP. The enzyme can unwind substrate with a single-stranded region 3' but not 5' of a double-stranded region, and can also anneal complementary single strands. The enzyme shows no activity in the absence of nucleotide cofactor or in the presence of ADP or the nonhydrolyzable homolog AMPPNP | Drosophila melanogaster | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3' to 5' helicase | - |
Drosophila melanogaster |
| RecQ4 | - |
Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Drosophila melanogaster | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | homozygous null mutants do not survive past the first instar because of severe defects in replication | Drosophila melanogaster |
| physiological function | the enzyme plays an essential role in replication | Drosophila melanogaster |
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