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Literature summary for 5.6.2.4 extracted from

  • Rizvi, I.; Choudhury, N.R.; Tuteja, N.
    Arabidopsis thaliana MCM3 single subunit of MCM2-7 complex functions as 3' to 5' DNA helicase (2016), Protoplasma, 253, 467-475 .
    View publication on PubMed
Search for more literature for 5.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene MCM3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged MCM3 protein in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
KCl required, the optimal salt concentration for activity is 50 mM. Salt concentrations of 150 mM and 200 mM are completely inhibitory to the helicase activity Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
KCl required, the optimal salt concentration for activity is 50 mM. Salt concentrations of 150 mM and 200 mM are completely inhibitory to the helicase activity Arabidopsis thaliana
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Arabidopsis thaliana
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FL33
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MCM3 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Arabidopsis thaliana ADP + phosphate
-
 ?
ATP + H2O AtMCM3 preferentially utilize ATP and dATP Arabidopsis thaliana ADP + phosphate
-
 ?
dATP + H2O AtMCM3 preferentially utilize ATP and dATP Arabidopsis thaliana dADP + phosphate
-
 ?
additional information MCM3 binds ATP and possess ATPase and ATP-dependent helicase activities. AtMCM3 shows a greater unwinding activity with 5' forked partial DNA duplex substrate as compared to 3' forked and non-forked substrates. ATP and magnesium ion are indispensable for its DNA helicase activity. Specifically, ATP and dATP are the preferred nucleotides for its unwinding activity. The directionality of the AtMCM3 is in 3' to 5' direction. The helicase activity is determined by strand displacement assay using oligomeric ds DNA substrate. Other nucleotides such as CTP, GTP, UTP, dCTP, dGTP, and dTTP do not support DNA unwinding by AtMCM3. Thus, the AtMCM3 specifically utilizes only ATP or dATP for the in vitro helicase activity Arabidopsis thaliana ?
-
-

Subunits

Subunits Comment Organism
multimer Arabidopsis thaliana MCM3 forms multimeric complexes Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
3' to 5' DNA helicase
-
 Arabidopsis thaliana
AtMCM3
-
 Arabidopsis thaliana
DNA replication licensing factor MCM3 UniProt Arabidopsis thaliana
MCM2-7
-
 Arabidopsis thaliana
MCM3
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the formation of prereplicative complex (pre-RC) is a requisite for the DNA replication initiation in eukaryotes. The pre-RC is composed of several replication factors which includes origin recognition complex (ORC), cell division cycle 6 (Cdc6), Cdc10-dependent transcript 1 (Cdt1), and minichromosome maintenance 2-7 (MCM2-7) complex. The subcomplex MCM4/6/7 possess in vitro helicase activity, while the MCM3 single subunit shows in vitro unwinding and ATPase activities Arabidopsis thaliana
physiological function the MCM3 single subunit of MCM2-7 complex functions as 3' to 5' DNA helicase Arabidopsis thaliana