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Literature summary for 5.6.2.4 extracted from

  • Suntornthiticharoen, P.; Srila, W.; Chavalitshewinkoon-Petmitr, P.; Limudomporn, P.; Yamabhai, M.
    Characterization of recombinant malarial RecQ DNA helicase (2014), Mol. Biochem. Parasitol., 196, 41-44.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, recombinant expression of C-terminally His10-tagged PfRecQ1 in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
daunorubicin inhibit the unwinding activity Plasmodium falciparum
doxorubicin inhibits the unwinding activity Plasmodium falciparum
additional information no inhibition of unwinding activity by aphidicolin, genistein, mitoxantrone, and netropsin Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
x * 87000, recombinant His10-tagged enzyme, SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Plasmodium falciparum
-
ADP + phosphate
-
?
additional information Plasmodium falciparum the enzyme efficiently unwinds partial duplex DNA substrate in a 3' to 5' direction, but the enzyme cannot unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity is driven by the hydrolysis of ATP. The helicase reaction requires the presence of ATP as a cofactor, although dATP supports the unwinding at 89% of the efficiency of ATP ?
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8I2W7 a multi-drug resistant strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Plasmodium falciparum ADP + phosphate
-
?
additional information the enzyme efficiently unwinds partial duplex DNA substrate in a 3' to 5' direction, but the enzyme cannot unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity is driven by the hydrolysis of ATP. The helicase reaction requires the presence of ATP as a cofactor, although dATP supports the unwinding at 89% of the efficiency of ATP Plasmodium falciparum ?
-
?

Subunits

Subunits Comment Organism
? x * 87000, recombinant His10-tagged enzyme, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
PfRecQ1
-
Plasmodium falciparum
RecQ DNA helicase
-
Plasmodium falciparum