Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, recombinant expression of C-terminally His10-tagged PfRecQ1 in Escherichia coli | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
daunorubicin | inhibit the unwinding activity | Plasmodium falciparum | |
doxorubicin | inhibits the unwinding activity | Plasmodium falciparum | |
additional information | no inhibition of unwinding activity by aphidicolin, genistein, mitoxantrone, and netropsin | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
x * 87000, recombinant His10-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Plasmodium falciparum | - |
ADP + phosphate | - |
? | |
additional information | Plasmodium falciparum | the enzyme efficiently unwinds partial duplex DNA substrate in a 3' to 5' direction, but the enzyme cannot unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity is driven by the hydrolysis of ATP. The helicase reaction requires the presence of ATP as a cofactor, although dATP supports the unwinding at 89% of the efficiency of ATP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8I2W7 | a multi-drug resistant strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Plasmodium falciparum | ADP + phosphate | - |
? | |
additional information | the enzyme efficiently unwinds partial duplex DNA substrate in a 3' to 5' direction, but the enzyme cannot unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity is driven by the hydrolysis of ATP. The helicase reaction requires the presence of ATP as a cofactor, although dATP supports the unwinding at 89% of the efficiency of ATP | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 87000, recombinant His10-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
PfRecQ1 | - |
Plasmodium falciparum |
RecQ DNA helicase | - |
Plasmodium falciparum |