Any feedback?
Literature summary for 5.6.2.4 extracted from
- Analysis of the crystal structure of an active MCM hexamer (2014), Elife, 3, e03433.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method at pH 7.6, crystal structure of a nearly full-length MCM hexamer that is helicase-active and thus has all features essential for unwinding DNA. The structure is a chimera of Sulfolobus solfataricus N-terminal domain and Pyrococcus furiosus ATPase domain. The chimera protein construct consists of SsoMCMN, (SsoMCM aa 1269) fused to PfMCMAAA (PfMCM aa 257361/729966 = aa 257966 with its intein, aa 362728, removed) | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q9UXG1 | - |
- |
| Saccharolobus solfataricus P2 | Q9UXG1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCM helicase | - |
Saccharolobus solfataricus |
| SSO0774 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 69 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Saccharolobus solfataricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
