Literature summary for 5.6.2.4 extracted from

Brewster, A.S.; Wang, G.; Yu, X.; Greenleaf, W.B.; Carazo, J.M.; Tjajadi, M.; Klein, M.G.; Chen, X.S.
Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase (2008), Proc. Natl. Acad. Sci. USA, 105, 20191-20196.

Search for more literature for 5.6.2.4

Crystallization (Commentary)

Crystallization (Comment)	Organism
4.35 A crystal structure of the near-full-length enzyme	Saccharolobus solfataricus
4.35 A crystal structure of the near-full-length enzyme. The structure shows an elongated fold, with 5 subdomains that are organized into 2 large N- and C-terminal domains	Saccharolobus solfataricus
4.35 A crystal structure of the near-full-length ssoMCM. The structure shows an elongated fold, with 5 subdomains that are organized into two large N- and C-terminal domains	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
A416R/A420R	mutant shows no helicase activity	Saccharolobus solfataricus
A416R/A420R	near abrogation of helicase activity	Saccharolobus solfataricus
A416R/A420R	no helicase activity detectable, wild-type protein exists as hexamers, mutants protein elutes predominantly in the monomer peak	Saccharolobus solfataricus
E202G/E203G/V204G	mutant shows no helicase activity	Saccharolobus solfataricus
E326A/D327A/R329A	mutant shows no helicase activity	Saccharolobus solfataricus
I555D/L556S/I557D	mutant shows no helicase activity	Saccharolobus solfataricus
L189D/D191R	mutant shows no helicase activity	Saccharolobus solfataricus
L189D/D191R	near abrogation of helicase activity	Saccharolobus solfataricus
L189D/D191R	no helicase activity detectable, wild-type protein exists as hexamers, mutants protein elutes predominantly in the monomer peak	Saccharolobus solfataricus
T550G/P551G/D552G/S553G/P554G	mutant shows no helicase activity	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00022	-	ATP	pH and temperature not specified in the publication, mutant enzyme E202G/E203G/V204G	Saccharolobus solfataricus
0.00028	-	ATP	pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.0004	-	ATP	pH and temperature not specified in the publication, mutant enzyme T550G/P551G/D552G/S553G/P554G	Saccharolobus solfataricus
0.0012	-	ATP	pH and temperature not specified in the publication, mutant enzyme L189D/D191R	Saccharolobus solfataricus
0.0016	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme E202G/E203G/V204G	Saccharolobus solfataricus
0.0019	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.0025	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme T550G/P551G/D552G/S553G/P554G	Saccharolobus solfataricus
0.0044	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A/D327A/R329A	Saccharolobus solfataricus
0.013	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme L189D/D191R	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
77428	-	6 * 77428, calculated from sequence, wild-type enzyme, critical roles for hexamerization and helicase function is demonstated	Saccharolobus solfataricus
462000	-	calculated from sequence	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q9UXG1	-	-
Saccharolobus solfataricus P2	Q9UXG1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Saccharolobus solfataricus	ADP + phosphate	-	?
ATP + H2O	-	Saccharolobus solfataricus P2	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 77428, calculated from sequence, wild-type enzyme, critical roles for hexamerization and helicase function is demonstated	Saccharolobus solfataricus
homohexamer	-	Saccharolobus solfataricus
homohexamer	critical roles for hexamerization and helicase function	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
MCM protein	-	Saccharolobus solfataricus
minichromosome maintenance protein	-	Saccharolobus solfataricus
SSO0774	locus name	Saccharolobus solfataricus
SsoMCM	-	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	ATP	pH and temperature not specified in the publication, mutant enzyme L189D/D191R	Saccharolobus solfataricus
0.045	-	ATP	pH and temperature not specified in the publication, mutant enzyme E326A/D327A/R329A	Saccharolobus solfataricus
0.047	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme I555D/L556S/I557D	Saccharolobus solfataricus
0.047	-	ATP	pH and temperature not specified in the publication, mutant enzyme E202G/E203G/V204G	Saccharolobus solfataricus
0.052	-	ATP	pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.052	-	ATP	pH and temperature not specified in the publication, mutant enzyme T550G/P551G/D552G/S553G/P554G	Saccharolobus solfataricus
0.055	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme E202G/E203G/V204G	Saccharolobus solfataricus
0.068	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus
0.09	-	ATP	+ Y-DNA, pH and temperature not specified in the publication, mutant enzyme L189D/D191R	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
25	-	ATP	pH and temperature not specified in the publication, mutant enzyme L189D/D191R	Saccharolobus solfataricus
185.7	-	ATP	pH and temperature not specified in the publication, wild-type enzyme	Saccharolobus solfataricus

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Release 2023.1 (January 2023)