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Literature summary for 5.6.2.4 extracted from

  • Graham, B.W.; Schauer, G.D.; Leuba, S.H.; Trakselis, M.A.
    Steric exclusion and wrapping of the excluded DNA strand occurs along discrete external binding paths during MCM helicase unwinding (2011), Nucleic Acids Res., 39, 6585-6595.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
K323A reduced helicase activity Saccharolobus solfataricus
K323A/R440A reduced helicase activity Saccharolobus solfataricus
R440A reduced helicase activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UXG1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O a steric exclusion and wrapping (SEW) model for MCM helicases is proposed, which the hexamer complex is stabilized by wrapping of the displaced 5'-strand around the exterior surface, resembling a spool of thread Saccharolobus solfataricus ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
MCM helicase
-
Saccharolobus solfataricus
minichromosome maintenance helicase
-
Saccharolobus solfataricus
SSO0774 locus name Saccharolobus solfataricus
SsoMCM
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharolobus solfataricus