Literature summary for 5.6.2.4 extracted from

Pucci, B.; De Felice, M.; Rossi, M.; Onesti, S.; Pisani, F.M.
Amino acids of the Sulfolobus solfataricus mini-chromosome maintenance-like DNA helicase involved in DNA binding/remodeling (2004), J. Biol. Chem., 279, 49222-49228.

Search for more literature for 5.6.2.4

Protein Variants

Protein Variants	Comment	Organism
H146A	mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity	Saccharolobus solfataricus
H146A	the purified mutant enzyme retains full ATPase activity	Saccharolobus solfataricus
K129A	mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity	Saccharolobus solfataricus
K129A	the purified mutant enzyme retains full ATPase activity	Saccharolobus solfataricus
K134A	mutation affects binding to duplex DNA molecules, whereas it has no effect on binding to single-stranded DNA and on the DNA unwinding activity	Saccharolobus solfataricus
K134A	the purified mutant enzyme retains full ATPase activity	Saccharolobus solfataricus
K194A	mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity	Saccharolobus solfataricus
K194A	the purified mutant enzyme retains full ATPase activity	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-
Saccharolobus solfataricus	Q9UXG1	-	-
Saccharolobus solfataricus P2	Q9UXG1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	DNA duplexes that contains a 30-nucleotide 5'-tail. The ability of the enzyme to bind single- but not double-stranded DNA is required for the unwinding function	Saccharolobus solfataricus	ADP + phosphate	-	?
ATP + H2O	DNA duplexes that contains a 30-nucleotide 5'-tail. The ability of the enzyme to bind single- but not double-stranded DNA is required for the unwinding function	Saccharolobus solfataricus P2	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
MCM-like DNA helicase	-	Methanothermobacter thermautotrophicus
MCM-like DNA helicase	-	Saccharolobus solfataricus
mini-chromosome maintenance-like DNA helicase	-	Methanothermobacter thermautotrophicus
mini-chromosome maintenance-like DNA helicase	-	Saccharolobus solfataricus
SsoMCM	-	Methanothermobacter thermautotrophicus
SsoMCM	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	assay at	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharolobus solfataricus

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Release 2023.1 (January 2023)