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Literature summary for 5.6.2.4 extracted from

  • Carpentieri, F.; de Felice, M.; de Falco, M.; Rossi, M.; Pisani, F.M.
    Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus (2002), J. Biol. Chem., 277, 12118-12127.
    View publication on PubMed
Search for more literature for 5.6.2.4

Activating Compound

Activating Compound Comment Organism Structure
single-stranded DNA binding protein stimulates activity and forms a protein/protein complex with the mini-chromosome maintenance protein helicase Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
K346A ATPase activity of the mutant is noticeably reduced, although not completely abolished, it is almost completely devoid of helicase activity Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 6 * 75000, 77428, calculated from sequence Saccharolobus solfataricus
470000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UXG1
-
-
Saccharolobus solfataricus P2 Q9UXG1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O ATP-dependent 3'-5' DNA helicase activity in vitro. Preferentially acts on DNA duplexes containing a 5'-tail Saccharolobus solfataricus ADP + phosphate
-
 ?
ATP + H2O dATP and ATP support DNA unwinding reaction Saccharolobus solfataricus ADP + phosphate
-
 ?
ATP + H2O ATP-dependent 3'-5' DNA helicase activity in vitro. Preferentially acts on DNA duplexes containing a 5'-tail Saccharolobus solfataricus P2 ADP + phosphate
-
 ?
ATP + H2O dATP and ATP support DNA unwinding reaction Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 75000, 77428, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
MCM protein
-
 Saccharolobus solfataricus
mini-chromosome maintenance protein
-
 Saccharolobus solfataricus
Sso MCM
-
 Saccharolobus solfataricus
SSO0774 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus