Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.4 extracted from

  • Pike, A.C.; Shrestha, B.; Popuri, V.; Burgess-Brown, N.; Muzzolini, L.M.; Costantini, S.; Vindigni, A.; Gileadi, O.
    Structure of the human RECQ1 helicase reveals a putative strand-separation pin (2009), Proc. Natl. Acad. Sci. USA, 106, 1039-1044.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
a construct (RECQ1(T1)) encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa is produced in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified RECQ1T1 protein is crystallized in the presence of ATP-gammaS and oligonucleotides by vapor diffusion from sitting drops equilibrated against 0.2 M sodium bromide, 20% PEG 3350, 10% ethylene glycol, 0.1 M bis-Tris propane (pH 7.5). Crystal structure of a truncated form (RECQ1(T1)) of the human RECQ1 protein with MgADP2- Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ATP mutant (RECQ1(T1))Y564A, a construct encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa Homo sapiens
0.115
-
 ATP full-length RECQ1 helicase Homo sapiens
0.135
-
 ATP (RECQ1(T1)), a construct encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P46063
-
-

Purification (Commentary)

Purification (Comment) Organism
a construct (RECQ1(T1)) encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa is produced in Escherichia coli and purified to more than 95% homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 ATP full-length RECQ1 helicase Homo sapiens
7.41
-
 ATP mutant (RECQ1(T1))Y564A, a construct encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa Homo sapiens
11.25
-
 ATP (RECQ1(T1)), a construct encompassing amino acids 49–616 (of 649) of RECQ1, followed by a C-terminal tag of 22 aa Homo sapiens