Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.4 extracted from

  • Tuteja, N.; Ochem, A.; Taneja, P.; Tuteja, R.; Skopac, D.; Falaschi, A.
    Purification and properties of human DNA helicase VI (1995), Nucleic Acids Res., 23, 2457-2463.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.4

General Stability

General Stability Organism
enzyme activity is destroyed if trypsin is included in the reaction Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, complete inhibition Homo sapiens
KCl optimal concentration: 100 mM. Inhibition at 200 mM Homo sapiens
Mg2+ required, optimal concentration: 0.8 mM. Inhibition at 4 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration: 100 mM. Inhibition at 200 mM Homo sapiens
Mg2+ required for maximal activity, optimal concentration: 0.8 mM. Inhibition at 4 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
128000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, DNA helicase VI loses 90% of its activity in 24 h Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O ATP-dependent DNA unwinding enzyme. HDH VI unwinds exclusively DNA duplexes with an annealed portion smaller than32 bp and prefers a replication fork-like structure of the substrate. It cannot unwind blunt-end duplexes and is inactive also on DNA-RNA or RNA-RNA hybrids. HDH VI unwinds DNA unidirectionally by moving in the 3' to 5' direction along the bound strand. ATP and dATP are equally good substrates Homo sapiens ADP + phosphate
-
 ?
dATP + H2O ATP-dependent DNA unwinding enzyme. HDH VI unwinds exclusively DNA duplexes with an annealed portion smaller than32 bp and prefers a replication fork-like structure of the substrate. It cannot unwind blunt-end duplexes and is inactive also on DNA-RNA or RNA-RNA hybrids. HDH VI unwinds DNA unidirectionally by moving in the 3' to 5' direction along the bound strand. ATP and dATP are equally good substrates Homo sapiens dADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 128000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DNA helicase VI
-
 Homo sapiens
HDH IV
-
 Homo sapiens