Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.4 extracted from

  • Turchi, J.J.; Murante, R.S.; Bambara, R.A.
    DNA substrate specificity of DNA helicase E from calf thymus (1992), Nucleic Acids Res., 20, 6075-6080.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.4

Activating Compound

Activating Compound Comment Organism Structure
replication protein A stimulates activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
additional information Hel E is inhibited by replication fork structures Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
thymus calf Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O the helicase is capable of displacing DNA fragments up to 140 nucleotides in length, but is unable to displace a DNA fragment 322 nucleotides in length. Preference for displacing primers whose 5' terminus is fully annealed as opposed to primers with a 12 nucleotide 5' unannealed tail. The presence of a 12 nucleotide 3' tail has no effect on the rate of displacement. DNA helicase E is capable of displacing a primer downstream of either a four nucleotide gap, a one nucleotide gap or a nick in the DNA substrate. Helicase E is inactive on a fully duplex DNA 30 base pairs in length Bos taurus ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DNA helicase E
-
 Bos taurus
Hel E
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bos taurus