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Literature summary for 5.6.2.4 extracted from

  • Smith, R.H.; Kotin, R.M.
    The Rep52 gene product of adeno-associated virus is a DNA helicase with 3'-to-5' polarity (1998), J. Virol., 72, 4874-4881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.4

Protein Variants

Protein Variants Comment Organism
K116H an MBP-Rep52 chimera bearing K116H mutation within a consensus helicase- and ATPase-associated motif (motif I or Walker A site) is deficient for both DNA helicase and ATPase activities adeno-associated virus 2
K340H in a Rep78 A-site mutant protein bearing mutation K340H, the MBP-Rep52 A-site mutant protein fails to exhibit a trans-dominant negative effect when it is mixed with wild-type MBP-Rep52 or MBP-Rep78 in vitro adeno-associated virus 2

Inhibitors

Inhibitors Comment Organism Structure
NaCl optimal concentration is 50-100 mM, higher concentrations inhibit helicase activity adeno-associated virus 2

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required, optimal activity at 4 mM MgCl2 adeno-associated virus 2
Mn2+ Mg2+ or Mn2+ required adeno-associated virus 2
NaCl optimal concentration is 50-100 mM, higher concentrations inhibit helicase activity adeno-associated virus 2

Organism

Organism UniProt Comment Textmining
adeno-associated virus 2 Q89270
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O DNA helicase with 3'-to-5' polarity. No helicase activity in absence of NTP adeno-associated virus 2 ADP + phosphate
-
 ?
CTP + H2O DNA helicase with 3'-to-5' polarity. No helicase activity in absence of NTP adeno-associated virus 2 CDP + phosphate
-
 ?
dATP + H2O DNA helicase with 3'-to-5' polarity. No helicase activity in absence of NTP. dATP is as efficient as ATP adeno-associated virus 2 dADP + phosphate
-
 ?
GTP + H2O DNA helicase with 3'-to-5' polarity. No helicase activity in absence of NTP adeno-associated virus 2 GDP + phosphate
-
 ?
additional information no helicase activity is observed with UTP, dCTP or dTTP, low levels of helicase activity is observed with dGTP adeno-associated virus 2 ?
-
 ?

Subunits

Subunits Comment Organism
More posttranslational modifications lacking in in vitro- or bacterially synthesized Rep52 may be required for efficient Rep52 multimerization adeno-associated virus 2

Synonyms

Synonyms Comment Organism
Rep52
-
 adeno-associated virus 2