Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and site-directed mutants | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
crystals are grown from hanging-drops | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
K52A | mutant is unable to function as a helicase | Saccharolobus solfataricus |
K646stop | mutant lacking domain 5 shows significantly faster rates of DNA unwinding than the wild-type protein | Saccharolobus solfataricus |
R255A | mutant shows significantly reduced helicase activity | Saccharolobus solfataricus |
R320A | mutant shows significantly reduced helicase activity | Saccharolobus solfataricus |
R662A | mutant lacking the first arginine of the RAR motif in domain 5 shows significantly faster rates of DNA unwinding than the wild-type protein | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | D0KN27 | - |
- |
Saccharolobus solfataricus 98/2 | D0KN27 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus 98/2 | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent DNA helicase Hel308 | - |
Saccharolobus solfataricus |
Hel308 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the assay is carried out at either 60°C or 45°C | Saccharolobus solfataricus |
60 | - |
the assay is carried out at either 60°C or 45°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | one function of the enzyme may be in the removal of bound proteins at stalled replication forks and recombination intermediates | Saccharolobus solfataricus |