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Literature summary for 5.6.2.4 extracted from

  • Richards, J.D.; Johnson, K.A.; Liu, H.; McRobbie, A.M.; McMahon, S.; Oke, M.; Carter, L.; Naismith, J.H.; White, M.F.
    Structure of the DNA repair helicase Hel308 reveals DNA binding and autoinhibitory domains (2008), J. Biol. Chem., 283, 5118-5126.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, wild-type and site-directed mutants Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are grown from hanging-drops Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
K52A mutant is unable to function as a helicase Saccharolobus solfataricus
K646stop mutant lacking domain 5 shows significantly faster rates of DNA unwinding than the wild-type protein Saccharolobus solfataricus
R255A mutant shows significantly reduced helicase activity Saccharolobus solfataricus
R320A mutant shows significantly reduced helicase activity Saccharolobus solfataricus
R662A mutant lacking the first arginine of the RAR motif in domain 5 shows significantly faster rates of DNA unwinding than the wild-type protein Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus D0KN27
-
-
Saccharolobus solfataricus 98/2 D0KN27
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Saccharolobus solfataricus ADP + phosphate
-
?
ATP + H2O
-
Saccharolobus solfataricus 98/2 ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
ATP-dependent DNA helicase Hel308
-
Saccharolobus solfataricus
Hel308
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
the assay is carried out at either 60°C or 45°C Saccharolobus solfataricus
60
-
the assay is carried out at either 60°C or 45°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function one function of the enzyme may be in the removal of bound proteins at stalled replication forks and recombination intermediates Saccharolobus solfataricus