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Literature summary for 5.6.2.4 extracted from

  • Karow, J.K.; Chakraverty, R.K.
    Hickson, I.D.: The Bloom's syndrome gene product is a 3'-5' DNA helicase (1997), J. Biol. Chem., 272, 30611-30604.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Double-stranded DNA the enzyme is strongly stimulated by either single- or double-stranded DNA Homo sapiens
single-stranded DNA the enzyme is strongly stimulated by either single- or double-stranded DNA Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of an oligohistidine-tagged version of the BLM gene product in Saccharomyces cerevisiae Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
x * 180000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
B-cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O the enzyme unwinds DNA in the 3'-5' direction with respect to the strand to which the enzyme is bound Homo sapiens ADP + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 180000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
BLM
-
Homo sapiens
BLM protein
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens