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Literature summary for 5.6.2.3 extracted from

  • Zhang, H.; Zhang, Z.; Yang, J.; He, Z.G.
    Functional characterization of DnaB helicase and its modulation by single-stranded DNA binding protein in Mycobacterium tuberculosis (2014), FEBS J., 281, 1256-1266.
    View publication on PubMed
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
single-stranded binding protein MtbSSB, physical interaction between single-stranded binding protein of Mycobacterium tuberculosis, MtbSSB, and MtbDnaB. The helicase activity of MtbDnaB is stimulated by MtbSSB at low concentrations and inhibited at high concentrations. An MtbSSB mutant with decreased binding affinity for ssDNA can stimulate the helicase activity of MtbDnaB over a wider concentration range than wild-type MtbSSB suggesting that MtbSSB assists in the loading of MtbDnaB on the DNA replication fork Mycobacterium tuberculosis
ssDNA the ATPase activity of enzyme DnaB is stimulated in the presence of ssDNA Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
gene dnaB, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information construction of an intein-deleted form of DnaB Mycobacterium tuberculosis
R227C site-directed mutagenesis, mutation in the ATP binding site ARPGVGKS Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
single-stranded binding protein MtbSSB, physical interaction between single-stranded binding protein of Mycobacterium tuberculosis, MtbSSB, and MtbDnaB. The helicase activity of MtbDnaB is stimulated by MtbSSB at low concentrations and inhibited at high concentrations. ssDNA-stimulated ATPase activity of DnaB can be inhibited in the presence of SSB, possibly due to the inability of DnaB to bind to the SSB-bound ssDNA Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Mycobacterium tuberculosis
-
 ADP + phosphate
-
 ?
additional information Mycobacterium tuberculosis the enzyme DnaB exhibits strong 5' to 3' helicase and ATPase activities ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 gene dnaB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and intein-deleted mutant enzymes fromn Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nicke affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Mycobacterium tuberculosis ADP + phosphate
-
 ?
additional information the enzyme DnaB exhibits strong 5' to 3' helicase and ATPase activities Mycobacterium tuberculosis ?
-
 ?

Synonyms

Synonyms Comment Organism
DnaB helicase
-
 Mycobacterium tuberculosis
MtbDnaB
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
additional information an MtbSSB mutant with decreased binding affinity for ssDNA can stimulate the helicase activity of MtbDnaB over a wider concentration range than wild-type MtbSSB suggesting that MtbSSB assists in the loading of MtbDnaB on the DNA replication fork Mycobacterium tuberculosis
physiological function helicase DnaB is important in the initiation and extension stages of DNA replication. DnaB proteins also exhibit ATPase activity, which provides the energy necessary for their helicase activity, the helicase activity of MtbDnaB has 5' to 3 polarity. The ATPase activity of DnaB is stimulated in the presence of ssDNA Mycobacterium tuberculosis