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Literature summary for 5.6.2.3 extracted from
- Human mitochondrial DNA helicase TWINKLE is both an unwinding and annealing helicase (2012), J. Biol. Chem., 287, 14545-14556.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K421A | the Walker A mutant K421A of TWINKLE fails to unwind dsDNA | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
UTP | pH 7.5, 30°, in the presence of forked DNA | Homo sapiens |  |
| 1.6 | - |
UTP | pH 7.5, 30°, in the presence of ssDNA | Homo sapiens | |
| 1.7 | - |
UTP | pH 7.5, 30°, in the presence of ssM13mp18 | Homo sapiens | |
| 3.4 | - |
UTP | pH 7.5, 30°, in the absence DNA | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgUTP | MgUTP stabilizes hexamers over higher oligomers | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | efficiency of unwinding is enhanced in the presence of a heterologous single strand-binding protein or a single-stranded (ss) DNA that is complementary to the unwound strand. TWINKLE has an antagonistic activity of annealing two complementary ssDNAs that interferes with unwinding in the absence of gp2.5 single strand-binding protein or ssDNA trap. Only ssDNA and not dsDNA competitively inhibits the annealing activity, although both DNAs bind with high affinities | Homo sapiens | ? | - |
? | |
| UTP + H2O | - |
Homo sapiens | UDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | recombinant TWINKLE assembles into hexamers and higher oligomers, and addition of MgUTP stabilizes hexamers over higher oligomers | Homo sapiens |
| oligomer | recombinant TWINKLE assembles into hexamers and higher oligomers, and addition of MgUTP stabilizes hexamers over higher oligomers | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TWINKLE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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