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Literature summary for 5.6.2.3 extracted from

  • Bean, D.W.; Kallam, W.E. Jr.; Matson, S.W.
    Purification and characterization of a DNA helicase from Saccharomyces cerevisiae (1993), J. Biol. Chem., 268, 21783-21790.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
single-stranded DNA-binding protein from Escherichia coli, strongly stimulates when long partial duplex substrates are used Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mn2+ can substitute for Mg2+, less effective Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
1 * 135000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O unwinds partial duplex DNA substrates, as long as 343 base pairs in length, in a reaction that is dependent on either ATP or dATP hydrolysis. The direction of the unwinding reaction is 5' to 3' with respect to the strand of DNA on which the enzyme is bound Saccharomyces cerevisiae ADP + phosphate
-
?
dATP + H2O unwinds partial duplex DNA substrates, as long as 343 base pairs in length, in a reaction that is dependent on either ATP or dATP hydrolysis. The direction of the unwinding reaction is 5' to 3' with respect to the strand of DNA on which the enzyme is bound Saccharomyces cerevisiae dADP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 135000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
scHelI
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
unwinding reaction Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity Saccharomyces cerevisiae