Activating Compound | Comment | Organism | Structure |
---|---|---|---|
single-stranded DNA-binding protein | from Escherichia coli, strongly stimulates when long partial duplex substrates are used | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mn2+ | can substitute for Mg2+, less effective | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
1 * 135000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | unwinds partial duplex DNA substrates, as long as 343 base pairs in length, in a reaction that is dependent on either ATP or dATP hydrolysis. The direction of the unwinding reaction is 5' to 3' with respect to the strand of DNA on which the enzyme is bound | Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
dATP + H2O | unwinds partial duplex DNA substrates, as long as 343 base pairs in length, in a reaction that is dependent on either ATP or dATP hydrolysis. The direction of the unwinding reaction is 5' to 3' with respect to the strand of DNA on which the enzyme is bound | Saccharomyces cerevisiae | dADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 135000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
scHelI | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
unwinding reaction | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity | Saccharomyces cerevisiae |