Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.3 extracted from

  • Ruiz-Maso, J.A.; Anand, S.P.; Espinosa, M.; Khan, S.A.; del Solar, G.
    Genetic and biochemical characterization of the Streptococcus pneumoniae PcrA helicase and its role in plasmid rolling circle replication (2006), J. Bacteriol., 188, 7416-7425.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
ssDNA stimulates ATPase activity Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Streptococcus pneumoniae PcrA is a chromosomally encoded DNA helicase of gram-positive bacteria involved in replication of rolling circle replicating plasmids ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q8DPU8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O PcrA is a chromosomally encoded DNA helicase of gram-positive bacteria involved in replication of rolling circle replicating plasmids Streptococcus pneumoniae ADP + phosphate
-
 ?
ATP + H2O hydrolyzes both ATP and dATP at similar levels. The enzyme shows 5' to 3' and 3' to 5' DNA helicase activities and binds efficiently to partially duplex DNA containing a hairpin structure adjacent to a 6-nucleotide 5' or 3' single-stranded tail and one unpaired (flap) nucleotide in the complementary strand Streptococcus pneumoniae ADP + phosphate
-
 ?
dATP + H2O hydrolyzes both ATP and dATP at similar levels. The enzyme shows 5' to 3' and 3' to 5' helicase activities and binds efficiently to partially duplex DNA containing a hairpin structure adjacent to a 6-nucleotide 5' or 3' single-stranded tail and one unpaired (flap) nucleotide in the complementary strand Streptococcus pneumoniae dADP + phosphate
-
 ?
additional information the enzyme is inefficient in in vitro replication of pT181, and perhaps as a consequence, this plasmid can not be established in Streptococcus pneumoniae Streptococcus pneumoniae ?
-
 ?

Synonyms

Synonyms Comment Organism
PcrA helicase
-
 Streptococcus pneumoniae
PcrASpn
-
 Streptococcus pneumoniae