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Literature summary for 5.6.2.2 extracted from
- Functional characterization and inhibition of the type II DNA topoisomerase coded by African swine fever virus (2016), Virology, 493, 209-216 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | at least 2 mM ATP is required for optimal activity | African swine fever virus |  |
| dithiothreitol | the optimum concentration for enzyme activity is 1 mM | African swine fever virus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | African swine fever virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | the use of KCl is optimal at a concentration of 75 mM | African swine fever virus | |
| Mg2+ | the enzyme requires Mg2+ at 6 mM for optimal activity. Magnesium can be substituted by Mn2+ (at least at 4 mM) or Cu2+ (at 2 mM) but not by Ca2+ or Zn2+ | African swine fever virus | |
| Na+ | the use of NaCl is optimal at a concentration of 75 mM | African swine fever virus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | African swine fever virus | - |
DNA + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| African swine fever virus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catenated kDNA + ATP + H2O | - |
African swine fever virus | decatenated kDNA + ADP + phosphate | - |
? | |
| DNA + ATP + H2O | - |
African swine fever virus | DNA + ADP + phosphate | - |
? | |
| additional information | the enzyme cannot supercoil relaxed DNA | African swine fever virus | ? | - |
? | |
| supercoiled pBR322 plasmid DNA + ATP + H2O | - |
African swine fever virus | relaxed pBR322 plasmid DNA + ADP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pP1192R | - |
African swine fever virus |
| type II DNA topoisomerase | - |
African swine fever virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
African swine fever virus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | the optimal temperature for activity ranges between 30 and 37°C, and while 50°C still sustains some activity, no activity is observed below 20°C | African swine fever virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
African swine fever virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | the activity of the enzyme is highest between pH 7.0 and 8.0, with 7.5 being optimal, but the enzyme is almost inactive at pH 9.0 | African swine fever virus |
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