Any feedback?
Literature summary for 5.6.2.2 extracted from
- Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI (2007), Nat. Struct. Mol. Biol., 14, 611-609.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus shibatae |
| expression in Escherichia coli | Methanosarcina mazei |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop method at 19°C, crystal structure is determined at 4.0 A resolution. The 220000 Da heterotetramer adopts a twin-gate architecture, in which a pair of ATPase domains at one end of the enzyme is poised to coordinate DNA movements into the enzyme and through a set of DNA-cleaving domains at the other end | Methanosarcina mazei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S424C | cysteine substitution mutant capable of forming an intersubunit disulfide bond upon ATP-induced dimerization. The mutant protein has a smaller radius of gyration (Rg) than the apoprotein. | Saccharolobus shibatae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
- |
Methanosarcina mazei |
| 42127 | - |
2 * 42127 (subunit A) + 2 * 68760, calculated from sequence | Methanosarcina mazei |
| 68760 | - |
2 * 42127 (subunit A) + 2 * 68760, calculated from sequence | Methanosarcina mazei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | Saccharolobus shibatae | the enzyme helps disentangle chromosomes to facilitate cell division | ? | - |
? |  |
| DNA + ATP + H2O | Methanosarcina mazei | the enzyme helps disentangle chromosomes to facilitate cell division | ? | - |
? | |
| DNA + ATP + H2O | Methanosarcina mazei DSM 3647 | the enzyme helps disentangle chromosomes to facilitate cell division | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | Q8PUB7 and Q8PUB8 | Q8PUB7: subunit A, Q8PUB8: subunit B | - |
| Methanosarcina mazei DSM 3647 | Q8PUB7 and Q8PUB8 | Q8PUB7: subunit A, Q8PUB8: subunit B | - |
| Saccharolobus shibatae | O05208 and O05207 | O05208: subunit A, O05207: subunit B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus shibatae |
- |
Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division | Saccharolobus shibatae | ? | - |
? | |
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division | Methanosarcina mazei | ? | - |
? | |
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission | Saccharolobus shibatae | ? | - |
? | |
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission | Methanosarcina mazei | ? | - |
? | |
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division | Methanosarcina mazei DSM 3647 | ? | - |
? | |
| DNA + ATP + H2O | the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission | Methanosarcina mazei DSM 3647 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 42127 (subunit A) + 2 * 68760, calculated from sequence | Methanosarcina mazei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MmT6 | - |
Methanosarcina mazei |
| SsT6 | - |
Saccharolobus shibatae |
| topo VI | - |
Saccharolobus shibatae |
| topo VI | - |
Methanosarcina mazei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme helps disentangle chromosomes to facilitate cell division | Saccharolobus shibatae |
| physiological function | the enzyme helps disentangle chromosomes to facilitate cell division | Methanosarcina mazei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
