Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | in the presence of a low amount of NaCl (10 mM), TopR2 is able to produce DNA with a very high number of positive superturns. At 100 mM NaCl TopR2 produces the highest amount of this highly positively supercoiled DNA. Further increase in NaCl concentration until 400 mM does not provide a further higher amount of positively supercoiled DNA. At 600 mM NaCl, the presence of some positively supercoiled topoisomers is still observed but no more at 800 mM | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
negatively supercoiled DNA + ATP + H2O | activity of TopR2 of is strictly dependent on the presence of ATP. The enzyme exhibits an high intrinsic processivity. It is able to introduce a very high number of positive superturns in DNA | Saccharolobus solfataricus | highly positively supercolied DNA + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Reverse gyrase | - |
Saccharolobus solfataricus |
TopR2 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | the temperature at which the higher amount of substrate is converted by TopR2 is 70 °C. Above 70 °C, the quantity of converted substrate progressively diminishes and is no more detectable at 90 °C. A decrease in temperature below 70 °C leads to a decrease in the amount of converted substrate. Second, the amount of positive supercoils introduced by TopR2 into the substrate is very high at all temperatures at which TopR2 is active (between 60 °C and 85 °C) | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharolobus solfataricus |