Cloned (Comment) | Organism |
---|---|
overexpression of both subunits in Escherichia coli | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45055 | - |
2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence | Saccharolobus shibatae |
60528 | - |
2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence | Saccharolobus shibatae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + ATP + H2O | Saccharolobus shibatae | the enzyme generates ATP-dependent double-strand breaks with two-nucleotide overhangs on supercoiled or linear DNA. topoVI is covalently attached to the 5'-ends of the broken DNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | O05207 and O05208 | O05207: subunit B, O05208: subunit A | - |
Purification (Comment) | Organism |
---|---|
purification procedure of a fully soluble recombinant topoVI is developed by expressing both subunits simultaneously in Escherichia coli | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + ATP + H2O | the enzyme generates ATP-dependent double-strand breaks with two-nucleotide overhangs on supercoiled or linear DNA. topoVI is covalently attached to the 5'-ends of the broken DNA | Saccharolobus shibatae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 45055 (subunit A) + 2 * 60528 (subunit B), calculated from sequence | Saccharolobus shibatae |
Synonyms | Comment | Organism |
---|---|---|
DNA topoisomerase VI | - |
Saccharolobus shibatae |
topoVI | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus shibatae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | the formation of the double-strand breaks on supercoiled or linear DNA is strictly dependent on the presence of ATP or adenosine 5'-(beta,gamma-imino)triphosphate. ATP binding is required to stabilize an enzyme conformation able to cleave the DNA backbone | Saccharolobus shibatae |