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Literature summary for 5.6.2.2 extracted from
- Reverse gyrase from Methanopyrus kandleri. Reconstitution of an active extremozyme from its two recombinant subunits (1997), J. Biol. Chem., 272, 13986-13990.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli. Reconstitution of the heterodimeric active reverse gyrase from the two recombinant proteins overexpressed in Escherichia coli and purification. Subunit RgyB has a DNA-dependent ATPase activity at high temperature (80 °C) and is independent of the presence of subunit RgyA. Subunit RgyA alone has no detectable activity. The addition of subunit RgyA to subunit RgyB reconstitutes positive supercoiling activity | Methanopyrus kandleri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
| 138000 | - |
1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanopyrus kandleri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanopyrus kandleri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | reconstitution of the heterodimeric active reverse gyrase from the two recombinant proteins overexpressed in Escherichia coli and purification. Subunit RgyB has a DNA-dependent ATPase activity at high temperature (80 °C) and is independent of the presence of subunit RgyA. Subunit RgyA alone has no detectable activity. The addition of subunit RgyA to subunit RgyB reconstitutes positive supercoiling activity | Methanopyrus kandleri | ? | - |
? |  |
| negatively supercoiled DNA + ATP + H2O | - |
Methanopyrus kandleri | positively supercoiled DNA + ADP + phosphate | - |
? | |
| relaxed DNA + ATP + H2O | - |
Methanopyrus kandleri | positively supercoiled DNA + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 138000 + 1 * 43000, the 138000 Da subunit is involved in the hydrolysis of ATP (RgyB) and the 43000 Da subunit forms the covalent complex with DNA during the topoisomerase reaction (RgyA) | Methanopyrus kandleri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-dependent type I 5'-topoisomerase | - |
Methanopyrus kandleri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Methanopyrus kandleri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Methanopyrus kandleri |
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Release 2023.1 (January 2023)
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