Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant topo IIbeta proteins in Saccharomyces cerevisiae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of only Tyr656, but not Ser1395, Thr1326 or Ser1545, decreases topo IIbeta decatenation activity | Homo sapiens |
S1395A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
S1545A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
T1426A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
T1426A/S1545A | site-directed mutagenesis, phosphorylation site mutant that shows unaltered decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
Y656F | site-directed mutagenesis, phosphorylation site mutant that shows reduced decatenation activity compared to the wild-type topo IIbeta | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes topo IIalpha and beta | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | both human isozymes topo IIalpha and beta are phosphorylated. Phosphorylation sites of topo IIbeta are Tyr656, Ser1395, Thr1426 and Ser1545 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant topo IIbeta proteins from Saccharomyces cerevisiae by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HL-60 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate for topo IIbeta is kinetoplast DNA. Topoisomerase II catalyzes topological changes in DNA and decatenation reaction. Residue Tyr656 of topoisomerase IIbeta is important for its catalytic activity, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | homology modeling of the frame 449-1108 of topo IIbeta comprising Tyr656 and Tyr711 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
topo II | - |
Homo sapiens |
Topo IIalpha | - |
Homo sapiens |
Topo IIbeta | - |
Homo sapiens |
Topoisomerase II | - |
Homo sapiens |
topoisomerase IIbeta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | Topoisomerase II catalyzes topological changes in DNA | Homo sapiens |