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Literature summary for 5.6.2.2 extracted from

  • Gilroy, K.L.; Austin, C.A.
    The impact of the C-terminal domain on the interaction of human DNA topoisomerase II alpha and beta with DNA (2011), PLoS ONE, 6, e14693.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Activating Compound

Activating Compound Comment Organism Structure
additional information the strength of interaction of human topoisomerase IIbeta, but not topoisomerase IIalpha, with a DNA substrate is significantly increased in the absence of the C-terminal domain Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated topoisomerase II isozymes lacking the C-terminal domain, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the C-terminal domain of topoisomerase IIbeta acts as a negative regulator Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ supports strand passage by both of the human topoisomerase II isozymes Homo sapiens
Mg2+ supports strand passage by both of the human topoisomerase II isozymes Homo sapiens
Mn2+ supports strand passage by both of the human topoisomerase II isozymes Homo sapiens
additional information presence of metal ions has no effect on DNA substrate binding, role of divalent metal ions in strand passage activity, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the C-terminal domain of topoisomerase IIbeta but not topoisomerase IIalpha affects the binding of the enzyme to the DNA. It alters the enzyme's KD for DNA binding. Differential modes of action of the two isoforms in vivo, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes alpha and beta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the C-terminal domain of topoisomerase IIbeta but not topoisomerase IIalpha affects the binding of the enzyme to the DNA. It alters the enzyme's KD for DNA binding. Differential modes of action of the two isoforms in vivo, overview Homo sapiens ?
-
 ?
additional information the enzyme contains an active site tyrosine within the winged helix domain, role of the C-terminal domain in the topoisomerase II-DNA interaction, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer the enzyme contains an active site tyrosine within the winged helix domain, domains of full length topoisomerase II isozymes, overview Homo sapiens

Synonyms

Synonyms Comment Organism
DNA topoisomerase II alpha
-
 Homo sapiens
DNA topoisomerase II beta
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 DNA binding assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information passage of the full length and truncated enzymes in the presence of a number of supporting metal ions shows no difference in relative decatenation between isoforms Homo sapiens
physiological function type II DNA topoisomerases are essential, ubiquitous enzymes that act to relieve topological problems arising in DNA from normal cellular activity. Their mechanism of action involves the ATP-dependent transport of one DNA duplex through a transient break in a second DNA duplex; metal ions are essential for strand passage. The two isozymes have distinct roles in the cell, overview. The C-terminal domain of topoisomerase IIbeta acts as a negative regulator Homo sapiens