Literature summary for 5.6.2.2 extracted from

Obado, S.O.; Bot, C.; Echeverry, M.C.; Bayona, J.C.; Alvarez, V.E.; Taylor, M.C.; Kelly, J.M.
Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei (2011), Nucleic Acids Res., 39, 1023-1033.

Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression of C-terminally c-Myc-tagged Topo-IIalpha	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
additional information	the Topo-II decatenation reaction is completely abolished in Topo-IIalpha knockout cells, while this is not the case in Topo-IIbeta knockout cells. Expression of Topo-II can rescue the bloodstream form from the effects of Topo-IIalpha knockdown. Complementation of the Topo-IIalpha RNAi phenotype by expression of truncated versions of the Trypanosoma cruzi enzyme, overview	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
etoposide	mediates inhibition of the DNA ligation step in the Topo-II decatenation reaction	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
centromer	associated	Trypanosoma brucei	-	-
additional information	the enzyme contains a nuclear targeting sequence of 6 residues	Trypanosoma brucei	-	-

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	clone 221a	-
Trypanosoma brucei Lister 427	-	clone 221a	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme contains two putative sumoylation motifs, both at corresponding sites in the C-terminal region. Sumoylation of the enzyme is not required for centromere-specific cleavage activity, in contrast to other species	Trypanosoma brucei

Source Tissue

Source Tissue	Comment	Organism	Textmining
epimastigote	-	Trypanosoma brucei	-
additional information	bloodstream form	Trypanosoma brucei	-

Subunits

Subunits	Comment	Organism
More	the variable C-terminal region of topoisomerase-II has a major role in regulating biological function	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
topoisomerase-II	-	Trypanosoma brucei

General Information

General Information	Comment	Organism
malfunction	in bloodstream form Trypanosoma brucei, RNAi-mediated depletion of topoisomerase-IIalpha, but not topoisomerase-IIbeta, results in the abolition of centromere-localized activity and is lethal	Trypanosoma brucei
additional information	the variable carboxyl terminal region of topoisomerase-II has a major role in regulating biological function	Trypanosoma brucei
physiological function	topoisomerase-II is important for chromosome segregation and accumulates at centromeres during prometaphase, where it resolves the DNA catenations that represent the last link between sister chromatids	Trypanosoma brucei

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Release 2023.1 (January 2023)