Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
K423Q | the mutation almost completely abolishes the ability of Top2 to accomplish plasmid relaxation at all temperatures, Top2-K423Q is essentially dead for multiple strand passage events | Schizosaccharomyces pombe |
additional information | purified mutant Top2-191 shows a slower catalytic cycle than wild-type Top2 at permissive temperature. Top2-191 allele loses its decatenation activity at 36°C, causing chromosomal segregation defects, however, at 19°C, it gains a function that promotes chromosomal segregation, phenotypes, overview | Schizosaccharomyces pombe |
Y835F | the Top2-191-Y835F mutant completely suppresses taz1DELTA cold sensitivity, the cleavage-dead allele of Top2-191 favours the closed clamp as the relevant suppressing intermediate | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | the top2-191 allele acts in a dominant manner to suppress mutant taz1DELTA cold sensitivity. This suppression does not rely on the decatenation activity of Top2 and is independent of its DNA strand-passage activity. Taz1 binds telomeres, which promotes smooth replication fork progression through the repetitive telomeric sequences. The enhanced presence of reaction intermediates in which Top2 is clamped around DNA, promotes the removal of telomeric entanglements in vivo, independently of catalytic cycle completion. Modelling of how the clamped enzyme-DNA complex promotes proper chromosomal segregation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cleavage of supercoiled pBR322 DNA | Schizosaccharomyces pombe | ? | - |
? | |
additional information | the top2-191 allele acts in a dominant manner to suppress mutant taz1DELTA cold sensitivity. This suppression does not rely on the decatenation activity of Top2 and is independent of its DNA strand-passage activity. Taz1 binds telomeres, which promotes smooth replication fork progression through the repetitive telomeric sequences. The enhanced presence of reaction intermediates in which Top2 is clamped around DNA, promotes the removal of telomeric entanglements in vivo, independently of catalytic cycle completion. Modelling of how the clamped enzyme-DNA complex promotes proper chromosomal segregation, overview | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Top2 | - |
Schizosaccharomyces pombe |
Topoisomerase II | - |
Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schizosaccharomyces pombe |
General Information | Comment | Organism |
---|---|---|
physiological function | the decatenation activity of topoisomerase II is essential for chromosomal segregation in mitosis | Schizosaccharomyces pombe |