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Literature summary for 5.6.2.2 extracted from

  • Germe, T.; Miller, K.; Cooper, J.P.
    A non-canonical function of topoisomerase II in disentangling dysfunctional telomeres (2009), EMBO J., 28, 2803-2811.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
K423Q the mutation almost completely abolishes the ability of Top2 to accomplish plasmid relaxation at all temperatures, Top2-K423Q is essentially dead for multiple strand passage events Schizosaccharomyces pombe
additional information purified mutant Top2-191 shows a slower catalytic cycle than wild-type Top2 at permissive temperature. Top2-191 allele loses its decatenation activity at 36°C, causing chromosomal segregation defects, however, at 19°C, it gains a function that promotes chromosomal segregation, phenotypes, overview Schizosaccharomyces pombe
Y835F the Top2-191-Y835F mutant completely suppresses taz1DELTA cold sensitivity, the cleavage-dead allele of Top2-191 favours the closed clamp as the relevant suppressing intermediate Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe the top2-191 allele acts in a dominant manner to suppress mutant taz1DELTA cold sensitivity. This suppression does not rely on the decatenation activity of Top2 and is independent of its DNA strand-passage activity. Taz1 binds telomeres, which promotes smooth replication fork progression through the repetitive telomeric sequences. The enhanced presence of reaction intermediates in which Top2 is clamped around DNA, promotes the removal of telomeric entanglements in vivo, independently of catalytic cycle completion. Modelling of how the clamped enzyme-DNA complex promotes proper chromosomal segregation, overview ?
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?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleavage of supercoiled pBR322 DNA Schizosaccharomyces pombe ?
-
?
additional information the top2-191 allele acts in a dominant manner to suppress mutant taz1DELTA cold sensitivity. This suppression does not rely on the decatenation activity of Top2 and is independent of its DNA strand-passage activity. Taz1 binds telomeres, which promotes smooth replication fork progression through the repetitive telomeric sequences. The enhanced presence of reaction intermediates in which Top2 is clamped around DNA, promotes the removal of telomeric entanglements in vivo, independently of catalytic cycle completion. Modelling of how the clamped enzyme-DNA complex promotes proper chromosomal segregation, overview Schizosaccharomyces pombe ?
-
?

Subunits

Subunits Comment Organism
homodimer
-
Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Top2
-
Schizosaccharomyces pombe
Topoisomerase II
-
Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
ATP
-
Schizosaccharomyces pombe

General Information

General Information Comment Organism
physiological function the decatenation activity of topoisomerase II is essential for chromosomal segregation in mitosis Schizosaccharomyces pombe