Literature summary for 5.6.2.2 extracted from

Bender, R.P.; Ham, A.J.; Osheroff, N.
Quinone-induced enhancement of DNA cleavage by human topoisomerase IIalpha: adduction of cysteine residues 392 and 405 (2007), Biochemistry, 46, 2856-2864.

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Protein Variants

Protein Variants	Comment	Organism
C170A	potential site of quinone adduction. Mutant does not show reduced sensitivity to benzoquinone	Homo sapiens
C392A	potential site of quinone adduction. Mutant shows 50% reduced sensitivity to benzoquinone and displays 2fold faster rates of DNA religation than wild-type. Mutation does not affect the ability of benzoquinone to block the N-terinal gate of the enzyme	Homo sapiens
C405A	potential site of quinone adduction. Mutant shows 50% reduced sensitivity to benzoquinone and displays 2fold faster rates of DNA religation than wild-type. Mutation does not affect the ability of benzoquinone to block the N-terinal gate of the enzyme	Homo sapiens
C455A	potential site of quinone adduction. Mutant does not show reduced sensitivity to benzoquinone	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
benzoquinone	inhibits DNA religation and blocks N-terminal gate of the protein by cross-linking its two protomer subunits. Adduction at C392 and C405 of protein is important for its action	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform IIalpha	-

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Release 2023.1 (January 2023)