Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.2 extracted from

  • Makarevitch, I.; Somers, D.A.
    Purification and characterization of topoisomerase IIA from Arabidopsis thaliana (2005), Plant Sci., 168, 1023-1033.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
etoposide inhibits the enzyme at the DNA relaxation step Arabidopsis thaliana
m-AMSA inhibits the enzyme at the DNA relaxation step Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Arabidopsis thaliana 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
Cu2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
KCl required for DNA relaxation, optimal at 100-150 mM Arabidopsis thaliana
Mg2+ dependent on, preferred divalent cation, most active at 5.0-7.5 mM Arabidopsis thaliana
Mn2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
additional information no activity with Ca2+ Arabidopsis thaliana
NaCl required for DNA relaxation, optimal at 100-150 mM Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia, nuclear encoded enzyme
-

Purification (Commentary)

Purification (Comment) Organism
Topo IIA 1100fold from seeds in a 5-step process, to homogeneity Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
ATP-dependent breakage, passage and rejoining of double-stranded DNA relaxation mechanism Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
relaxation activity of the purified Topo IIA Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Arabidopsis thaliana ?
-
?
supercoiled dsDNA relaxation of supercoiled dsDNA, reaction mechanism Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
Topo IIA
-
Arabidopsis thaliana
topoisomerase IIA
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
purified Topo IIA is temperature-labile Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
purified Topo IIA is pH-labile Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana