Inhibitors | Comment | Organism | Structure |
---|---|---|---|
etoposide | inhibits the enzyme at the DNA relaxation step | Arabidopsis thaliana | |
m-AMSA | inhibits the enzyme at the DNA relaxation step | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute Mg2+ in a concentration range of 5-10 mM, less effective | Arabidopsis thaliana | |
Cu2+ | can substitute Mg2+ in a concentration range of 5-10 mM, less effective | Arabidopsis thaliana | |
KCl | required for DNA relaxation, optimal at 100-150 mM | Arabidopsis thaliana | |
Mg2+ | dependent on, preferred divalent cation, most active at 5.0-7.5 mM | Arabidopsis thaliana | |
Mn2+ | can substitute Mg2+ in a concentration range of 5-10 mM, less effective | Arabidopsis thaliana | |
additional information | no activity with Ca2+ | Arabidopsis thaliana | |
NaCl | required for DNA relaxation, optimal at 100-150 mM | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia, nuclear encoded enzyme | - |
Purification (Comment) | Organism |
---|---|
Topo IIA 1100fold from seeds in a 5-step process, to homogeneity | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP-dependent breakage, passage and rejoining of double-stranded DNA | relaxation mechanism | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relaxation activity of the purified Topo IIA | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity | Arabidopsis thaliana | ? | - |
? | |
supercoiled dsDNA | relaxation of supercoiled dsDNA, reaction mechanism | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Topo IIA | - |
Arabidopsis thaliana |
topoisomerase IIA | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
purified Topo IIA is temperature-labile | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
purified Topo IIA is pH-labile | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |