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Literature summary for 5.6.2.2 extracted from

  • Iwabata, K.; Koshiyama, A.; Yamaguchi, T.; Sugawara, H.; Hamada, F.N.; Namekawa, S.H.; Ishii, S.; Ishizaki, T.; Chiku, H.; Nara, T.; Sakaguchi, K.
    DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis (2005), Nucleic Acids Res., 33, 5809-5818.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Activating Compound

Activating Compound Comment Organism Structure
Lim15/Dmc1 from Coprimus cinereus, a meiosis-specific RecA-like protein, activates the DNA relaxation and decatenation activity of topoisomerase II in vitro, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview Coprinopsis cinerea

Cloned(Commentary)

Cloned (Comment) Organism
expression analysis of enzyme and protein Lim15 in vivo during meiosis, expression of Lim15 and topoisomerase II in the yeast two-hybrid system using Saccharomyces cerevisiae strain AH109, expression of His-tagged enzyme comprising residues 1066-1569 in Escherichia coli Coprinopsis cinerea

Inhibitors

Inhibitors Comment Organism Structure
Lim15/Dmc1 from Coprimus cinereus, a meiosis-specific RecA-like protein, Lim15 inhibits the topoisomerase II activity in vivo, but activates the DNA relaxation and decatenation activity of topoisomerase II in vitro Coprinopsis cinerea

Localization

Localization Comment Organism GeneOntology No. Textmining
chromosome co-localization with meiosis-specific RecA-like protein Lim15/Dmc1 from the leptotene to the zygotene stage Coprinopsis cinerea 5694
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for ATPase activity Coprinopsis cinerea
Mg2+
-
 Coprinopsis cinerea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coprinopsis cinerea DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview, Lim15 inhibits the topoisomerase II activity in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea Q5KSK9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme comprising residues 1066-1569 from Escherichia coli by nickel affinity chromatography Coprinopsis cinerea

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression analysis of enzyme and protein Lim15 in vivo during meiosis, overview Coprinopsis cinerea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded M13 DNA + ATP + H2O breakage, passage, and rejoining of double-stranded M13 DNA, including ATPase activity Coprinopsis cinerea ?
-
 ?
additional information DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview, Lim15 inhibits the topoisomerase II activity in vivo Coprinopsis cinerea ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase II
-
 Coprinopsis cinerea
TopII
-
 Coprinopsis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Coprinopsis cinerea

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Coprinopsis cinerea