Literature summary for 5.6.2.2 extracted from

Jain, P.; Nagaraja, V.
An atypical type II topoisomerase from Mycobacterium smegmatis with positive supercoiling activity (2005), Mol. Microbiol., 58, 1392-1405.

Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
enzyme is encoded by 2 orfs TopoM and TopoN, DNA sequence determination and analysis, phylogenetic analysis	Mycolicibacterium smegmatis

Inhibitors

Inhibitors	Comment	Organism	Structure
Ciprofloxacin	weak inhibition of TopoNM with IC50 of 0.125 mg/ml	Mycolicibacterium smegmatis
novobiocin	inhibition of TopoNM with IC50 of 0.075 mg/ml	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	encoded by 2 orfs TopoM and TopoN	-

Purification (Commentary)

Purification (Comment)	Organism
by ion exchange chromatography	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dsDNA + ATP + H2O	breakage, passage, decatenation, and rejoining of dsDNA	Mycolicibacterium smegmatis	?	-	?
additional information	the enzyme introduces positively supercoils in both relaxed and negatively supercoiled DNA in an ATP-dependent manner	Mycolicibacterium smegmatis	?	-	?
negatively supercoiled DNA + ATP + H2O	ATP-dependent relaxation of negatively supercoiled DNA	Mycolicibacterium smegmatis	?	-	?

Subunits

Subunits	Comment	Organism
More	domain organization	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
TopoNM	-	Mycolicibacterium smegmatis
type II topoisomerase	-	Mycolicibacterium smegmatis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)