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Literature summary for 5.6.2.2 extracted from
- Construction, characterization, and complementation of a conditional-lethal DNA topoisomerase IIalpha mutant human cell line (2004), Mol. Biol. Cell, 15, 5700-5711.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme is a target of numerous anti-tumor drugs | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GFP-tagged enzyme in mutant enzyme-depleted HTETOP cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction, characterization, and complementation of a conditional-lethal DNA enzyme mutant cell line HTETOP, in which over 95% of the enzyme expression can be silenced by tetracycline, e.g. doxycycline, HTETOP cells enter mitosis and undergo chromosome condensation, but with delayed kinetics, anaphase and cytokinesis are completely eliminated leading to cell death, overview, cells can be rescued by recombinant expression of GFP-tagged enzyme | Homo sapiens |
| S1106A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, during recombinant expression in enzyme-depleted HTETOP cells | Homo sapiens |
| S1247A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, during recombinant expression in enzyme-depleted HTETOP cells | Homo sapiens |
| S1354A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, during recombinant expression in enzyme-depleted HTETOP cells | Homo sapiens |
| S1393A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme, during recombinant expression in enzyme-depleted HTETOP cells | Homo sapiens |
| Y805F | site-directed mutagenesis, inactive mutant, no complementation of the enzyme-depleted HTETOP cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chromosome | enzyme is an abundant constituent of the mitotic chromosome | Homo sapiens | 5694 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme is important in chromosome segregation | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation at S1106, S1247, S1354, or S1393 is required for activity, but not essential | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP-dependent breakage, passage and rejoining of double-stranded DNA | active site Y805 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | decatenation of DNA | Homo sapiens | DNA + ADP + phosphate | - |
? | |
| additional information | the enzyme is important in chromosome segregation | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topoIIalpha | - |
Homo sapiens |
| topoisomerase IIalpha | - |
Homo sapiens |
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