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Literature summary for 5.6.2.2 extracted from
- Structure-activity study of the interaction of bioreductive benzoquinone alkylating agents with DNA topoisomerase II (2006), Cancer Chemother. Pharmacol., 57, 221-233.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of isozyme IIalpha in Saccharomyces cerevisiae | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-hydroquinone | inhibition mechanism | Homo sapiens |  |
| benzoquinone | alkylating agent, inhibits isozyme IIalpha via DNA cross-linking, forms cysteine sulfhydryl adducts, inhibits cell growth in vivo, which is 10fold increased in absence of cysteine, quantitative structure-activity analysis, structure of DNA binding, overview, inhibition mechanism | Homo sapiens | |
| benzoquinone mustard compounds | induce the formation of DNA-protein complexes, quantitative structure-activity analysis, inhibition mechanism, overview | Homo sapiens | |
| EO9 | inhibition mechanism | Homo sapiens | |
| etoposide | - |
Homo sapiens | |
| menadione | inhibition mechanism | Homo sapiens | |
| additional information | IC50 values, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme IIalpha | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| K-562 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pBR322 DNA + ATP + H2O | decatenation, relaxation and cleavage of pBR322 DNA | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA topoisomerase II | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | required for decatenation activity | Homo sapiens | |
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Release 2023.1 (January 2023)
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