Activating Compound | Comment | Organism | Structure |
---|---|---|---|
amsacrine | weak activation of DNA cleavage at some sites | Chlorella virus Marburg | |
etoposide | weak activation of DNA cleavage at some sites | Chlorella virus Marburg |
Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria chlorella virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amsacrine | weak inhibition of DNA cleavage at some sites, primarily inhibition of DNA ligation, low sensitivity for the drug | Chlorella virus Marburg | |
CP-115953 | - |
Chlorella virus Marburg | |
EDTA | - |
Chlorella virus Marburg | |
EDTA | - |
Paramecium bursaria chlorella virus | |
etoposide | weak inhibition of DNA cleavage at some sites, primarily inhibition of DNA ligation, low sensitivity for the drug | Chlorella virus Marburg |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Chlorella virus Marburg | |
KCl | - |
Paramecium bursaria chlorella virus | |
Mg2+ | required for cleavage | Chlorella virus Marburg | |
Mg2+ | required for cleavage | Paramecium bursaria chlorella virus | |
NaCl | - |
Paramecium bursaria chlorella virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + ATP + H2O | Paramecium bursaria chlorella virus | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for conrol of DNA topology | ? | - |
? | |
dsDNA + ATP + H2O | Chlorella virus Marburg | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for control of DNA topology | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus Marburg | - |
i.e. CVM-1 | - |
Paramecium bursaria chlorella virus | - |
i.e. PBCV-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for conrol of DNA topology | Paramecium bursaria chlorella virus | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for control of DNA topology | Chlorella virus Marburg | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity and high strand passage activity of the viral type enzyme, tight DNA binding, mapping of DNA cleavage sites | Chlorella virus Marburg | ? | - |
? | |
dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme, mapping of DNA cleavage sites | Paramecium bursaria chlorella virus | ? | - |
? | |
kinetoplast DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA is isolated from Crithidia fasciculata | Chlorella virus Marburg | ? | - |
? | |
additional information | formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome | Chlorella virus Marburg | ? | - |
? | |
additional information | formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome | Paramecium bursaria chlorella virus | ? | - |
? | |
negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, and rejoining of negatively supercoiled pBR322 DNA | Paramecium bursaria chlorella virus | ? | - |
? | |
negatively supercoiled pBR322 DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA | Chlorella virus Marburg | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Topoisomerase II | - |
Chlorella virus Marburg |
Topoisomerase II | - |
Paramecium bursaria chlorella virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Paramecium bursaria chlorella virus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Chlorella virus Marburg |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Chlorella virus Marburg |
8.5 | - |
assay at | Paramecium bursaria chlorella virus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Paramecium bursaria chlorella virus | |
ATP | dependent on, required for DNA relaxation and decatenation | Chlorella virus Marburg |