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Literature summary for 5.6.2.2 extracted from
- Cobalt enhances DNA cleavage mediated by human topoisomerase II alpha in vitro and in cultured cells (2004), Biochemistry, 43, 728-735.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| etoposide | anti-cancer drug, the enzyme inhibition by etoposide is not influenced by the divalent cation | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | slightly activating above 5 mM | Homo sapiens | |
| Ca2+ | activating at 1-5 mM | Homo sapiens | |
| Cd2+ | activates up to 1 mM, inhibits above 1 mM | Homo sapiens | |
| Co2+ | highly activating at 1-5 mM, enhances DNA cleavage mediated by topoisomerase II alpha in vitro and in cultured cells, 6-13fold higher activity in DNA cleavage compared to Mg2+, but reduced DNA ligation activity | Homo sapiens | |
| Mg2+ | slightly activating above 5 mM | Homo sapiens | |
| Mn2+ | highly activating at 1-5 mM | Homo sapiens | |
| additional information | activity with divalent cations, overview, the enzyme inhibition by etoposide is not influenced by the divalent cation | Homo sapiens | |
| Sr2+ | slightly activating above 5 mM | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | Homo sapiens | cleavage, relaxation, decatenation, and religation of DNA | DNA + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MCF-7 cell | breast adenocarcinoma cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + ATP + H2O | cleavage, relaxation, decatenation, and religation of DNA | Homo sapiens | DNA + ADP + phosphate | - |
? | |
| kinetoplast DNA + ATP + H2O | decatenation of kinetoplast DNA | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| topoisomerase II alpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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