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Literature summary for 5.6.2.2 extracted from

  • West, K.L.; Austin, C.A.
    Human DNA topoisomerase IIbeta binds and cleaves four-way junction DNA in vitro (1999), Nucleic Acids Res., 27, 984-992.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Metals/Ions

Metals/Ions Comment Organism Structure
additional information neither Mg2+ nor ca2+ are required for topoisomerase IIbeta binding to either the 40bp duplex or the four-way junction Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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 DNA topoisomerase IIbeta
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
four-way junction DNA + ATP + H2O binds and cleaves four-way junction DNA in vitro, topoisomerase IIbeta has a 4fold higher affinity for the four-way junction than for the linear duplex, the enzyme binds to the centre of the duplex, the four-way junction contains the sequence of a 40 bp linear duplex (containing a single topoisomerase cleavage site) along to adjacent arms, with the cleavage site straddling the point of strand exchange, the remaining two arms are comprised of sequences which do not contain topoisomerase cleavage sites, except that a potential m-AMSA-inducible cleavage site is introduced across the junction of these two arms Homo sapiens ?
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