Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-(9-acridinylamino)methansulfon-m-anisidide | - |
Crithidia fasciculata | |
Berenil | - |
Crithidia fasciculata | |
coumermycin | - |
Crithidia fasciculata | |
ellipticines | - |
Crithidia fasciculata | |
Ethidium bromide | - |
Crithidia fasciculata | |
etoposide | - |
Crithidia fasciculata | |
NEM | - |
Crithidia fasciculata | |
novobiocin | - |
Crithidia fasciculata | |
Teniposide | - |
Crithidia fasciculata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Crithidia fasciculata | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 | Crithidia fasciculata | |
Mg2+ | required | Crithidia fasciculata | |
Mg2+ | effective at 0.1-8 mM MgCl2 | Crithidia fasciculata | |
NaCl | requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 | Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
132000 | - |
2 * 132000, SDS-PAGE | Crithidia fasciculata |
264000 | - |
gel filtration, glycerol density gradient sedimentation | Crithidia fasciculata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Crithidia fasciculata | localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
novobiocin affinity purification | Crithidia fasciculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication | Crithidia fasciculata | ? | - |
? | |
network of DNA rings + ATP + H2O | decatenation | Crithidia fasciculata | monomeric DNA circles + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | catenation | Crithidia fasciculata | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation | Crithidia fasciculata | relaxed DNA + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | ATP-dependent relaxation | Crithidia fasciculata | relaxed DNA + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 132000, SDS-PAGE | Crithidia fasciculata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.8 | - |
Crithidia fasciculata |